MRI-guided stereotaxic brain surgery in the infant and adult common marmoset.
Nat Protoc
; 11(7): 1299-308, 2016 07.
Article
in En
| MEDLINE
| ID: mdl-27336707
ABSTRACT
In the past decade, the New World common marmoset (Callithrix jacchus) has taken a seminal position in neurobiological research, fueled in part by its smooth cortical sheet, which allows cortical areas to be easily accessed by current technologies on the dorsal surface of the brain. In this protocol, we describe a method for the precision placement of agents (e.g., tracers or neurotoxins) into small brain regions of the infant and adult marmoset, using an MRI-guided approach. This strategy uses a protocol for prolonged anesthesia without the need for intubation that we have recently developed, alongside appropriate analgesia and monitoring. The protocol can be readily adapted to be used together with advanced research techniques, such as two-photon microscopy and optical imaging. Including a 5-d postoperative care plan, this protocol takes 7 d to complete. The protocol requires a team of personnel experienced in marmoset care and handling, and small-animal neurosurgery; an assistant for monitoring the animal and assisting with anesthesia; and an MRI technician.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain
/
Magnetic Resonance Imaging
/
Surgery, Computer-Assisted
Type of study:
Guideline
Limits:
Animals
Language:
En
Journal:
Nat Protoc
Year:
2016
Document type:
Article
Affiliation country:
Australia