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Generation and characterization of human iPSC lines derived from a Primary Hyperoxaluria Type I patient with p.I244T mutation.
Zapata-Linares, Natalia; Rodriguez, Saray; Salido, Eduardo; Abizanda, Gloria; Iglesias, Elena; Prosper, Felipe; Gonzalez-Aseguinolaza, Gloria; Rodriguez-Madoz, Juan R.
Affiliation
  • Zapata-Linares N; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, IdiSNA, Pamplona, Spain.
  • Rodriguez S; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, IdiSNA, Pamplona, Spain.
  • Salido E; Hospital Universitario de Canarias, Universidad La Laguna, Centre for Biomedical Research on Rare Diseases (CIBERER), Tenerife, Spain.
  • Abizanda G; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, IdiSNA, Pamplona, Spain.
  • Iglesias E; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, IdiSNA, Pamplona, Spain.
  • Prosper F; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, IdiSNA, Pamplona, Spain; Area of Cell Therapy, Clínica Universidad de Navarra, University of Navarra, Instituto de Investigación Sanitaria de Navarra, IdiSNA, Pam
  • Gonzalez-Aseguinolaza G; Gene Therapy and Regulation of Gene Expression Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, IdiSNA, Pamplona, Spain. Electronic address: ggasegui@unav.es.
  • Rodriguez-Madoz JR; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, IdiSNA, Pamplona, Spain. Electronic address: jrrodriguez@unav.es.
Stem Cell Res ; 16(1): 116-9, 2016 Jan.
Article in En | MEDLINE | ID: mdl-27345794
ABSTRACT
In this work we describe for the first time the generation and characterization of human induced pluripotent stem cells (hiPSCs) from peripheral blood mononuclear cells (PBMCs) and dermal fibroblasts of a Primary Hyperoxaluria Type I (PH1)-diagnosed patient with p.I244T mutation, which is highly prevalent in Canary Islands due to founder effect. Cell reprogramming was performed using non-integrative Sendai viruses containing the Yamanaka factors and the generated PH1-hiPSC lines (PH1-PBMCs-hiPSC4F1 and PH1-Fib-hiPSC4F1) showed normal karyotypes, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers and in vivo differentiation ability to the three germ layers.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hyperoxaluria, Primary / Induced Pluripotent Stem Cells / Transaminases Limits: Humans / Male Language: En Journal: Stem Cell Res Year: 2016 Document type: Article Affiliation country: Spain
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hyperoxaluria, Primary / Induced Pluripotent Stem Cells / Transaminases Limits: Humans / Male Language: En Journal: Stem Cell Res Year: 2016 Document type: Article Affiliation country: Spain