Polymerase Interactions with Wobble Mismatches in Synthetic Genetic Systems and Their Evolutionary Implications.
Biochemistry
; 55(28): 3847-50, 2016 07 19.
Article
in En
| MEDLINE
| ID: mdl-27347689
ABSTRACT
In addition to completing the Watson-Crick nucleobase matching "concept" (big pairs with small, hydrogen bond donors pair with hydrogen bond acceptors), artificially expanded genetic information systems (AEGIS) also challenge DNA polymerases with a complete set of mismatches, including wobble mismatches. Here, we explore wobble mismatches with AEGIS with DNA polymerase 1 from Escherichia coli. Remarkably, we find that the polymerase tolerates an AEGISstandard wobble that has the same geometry as the GT wobble that polymerases have evolved to exclude but excludes a wobble geometry that polymerases have never encountered in natural history. These results suggest certain limits to "structural analogy" and "evolutionary guidance" as tools to help synthetic biologists expand DNA alphabets.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Evolution, Molecular
/
Base Pair Mismatch
/
DNA Polymerase I
Language:
En
Journal:
Biochemistry
Year:
2016
Document type:
Article
Affiliation country:
United States