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A Microbiome DNA Enrichment Method for Next-Generation Sequencing Sample Preparation.
Yigit, Erbay; Feehery, George R; Langhorst, Bradley W; Stewart, Fiona J; Dimalanta, Eileen T; Pradhan, Sriharsa; Slatko, Barton; Gardner, Andrew F; McFarland, James; Sumner, Christine; Davis, Theodore B.
Affiliation
  • Yigit E; New England Biolabs, Ipswich, Massachusetts.
  • Feehery GR; New England Biolabs, Ipswich, Massachusetts.
  • Langhorst BW; New England Biolabs, Ipswich, Massachusetts.
  • Stewart FJ; New England Biolabs, Ipswich, Massachusetts.
  • Dimalanta ET; New England Biolabs, Ipswich, Massachusetts.
  • Pradhan S; New England Biolabs, Ipswich, Massachusetts.
  • Slatko B; New England Biolabs, Ipswich, Massachusetts.
  • Gardner AF; New England Biolabs, Ipswich, Massachusetts.
  • McFarland J; New England Biolabs, Ipswich, Massachusetts.
  • Sumner C; New England Biolabs, Ipswich, Massachusetts.
  • Davis TB; New England Biolabs, Ipswich, Massachusetts.
Curr Protoc Mol Biol ; 115: 7.26.1-7.26.14, 2016 07 01.
Article in En | MEDLINE | ID: mdl-27366894
"Microbiome" is used to describe the communities of microorganisms and their genes in a particular environment, including communities in association with a eukaryotic host or part of a host. One challenge in microbiome analysis concerns the presence of host DNA in samples. Removal of host DNA before sequencing results in greater sequence depth of the intended microbiome target population. This unit describes a novel method of microbial DNA enrichment in which methylated host DNA such as human genomic DNA is selectively bound and separated from microbial DNA before next-generation sequencing (NGS) library construction. This microbiome enrichment technique yields a higher fraction of microbial sequencing reads and improved read quality resulting in a reduced cost of downstream data generation and analysis. © 2016 by John Wiley & Sons, Inc.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Sequence Analysis, DNA / High-Throughput Nucleotide Sequencing / Microbiota Limits: Humans Language: En Journal: Curr Protoc Mol Biol Year: 2016 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Sequence Analysis, DNA / High-Throughput Nucleotide Sequencing / Microbiota Limits: Humans Language: En Journal: Curr Protoc Mol Biol Year: 2016 Document type: Article Country of publication: United States