A Microbiome DNA Enrichment Method for Next-Generation Sequencing Sample Preparation.
Curr Protoc Mol Biol
; 115: 7.26.1-7.26.14, 2016 07 01.
Article
in En
| MEDLINE
| ID: mdl-27366894
"Microbiome" is used to describe the communities of microorganisms and their genes in a particular environment, including communities in association with a eukaryotic host or part of a host. One challenge in microbiome analysis concerns the presence of host DNA in samples. Removal of host DNA before sequencing results in greater sequence depth of the intended microbiome target population. This unit describes a novel method of microbial DNA enrichment in which methylated host DNA such as human genomic DNA is selectively bound and separated from microbial DNA before next-generation sequencing (NGS) library construction. This microbiome enrichment technique yields a higher fraction of microbial sequencing reads and improved read quality resulting in a reduced cost of downstream data generation and analysis. © 2016 by John Wiley & Sons, Inc.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Sequence Analysis, DNA
/
High-Throughput Nucleotide Sequencing
/
Microbiota
Limits:
Humans
Language:
En
Journal:
Curr Protoc Mol Biol
Year:
2016
Document type:
Article
Country of publication:
United States