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Contingent and non-contingent recreational-like exposure to ethanol alters BDNF expression and signaling in the cortico-accumbal network differently.
Orrù, Alessandro; Caffino, Lucia; Moro, Federico; Cassina, Chiara; Giannotti, Giuseppe; Di Clemente, Angelo; Fumagalli, Fabio; Cervo, Luigi.
Affiliation
  • Orrù A; Experimental Psychopharmacology, Department of Neuroscience, IRCCS-Istituto di Ricerche Farmacologiche 'Mario Negri', Via Giuseppe La Masa 19, 20156, Milan, Italy. alessandro.orru@ift.cnr.it.
  • Caffino L; Institute of Translational Pharmacology (C.N.R.), Parco Scientifico e Tecnologico della Sardegna, Polaris - Edificio 5 - Località, Piscinamanna, 09010, Pula, Cagliari, Italy. alessandro.orru@ift.cnr.it.
  • Moro F; Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via Balzaretti 9, 20133, Milan, Italy.
  • Cassina C; Experimental Psychopharmacology, Department of Neuroscience, IRCCS-Istituto di Ricerche Farmacologiche 'Mario Negri', Via Giuseppe La Masa 19, 20156, Milan, Italy.
  • Giannotti G; Experimental Psychopharmacology, Department of Neuroscience, IRCCS-Istituto di Ricerche Farmacologiche 'Mario Negri', Via Giuseppe La Masa 19, 20156, Milan, Italy.
  • Di Clemente A; Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via Balzaretti 9, 20133, Milan, Italy.
  • Fumagalli F; Experimental Psychopharmacology, Department of Neuroscience, IRCCS-Istituto di Ricerche Farmacologiche 'Mario Negri', Via Giuseppe La Masa 19, 20156, Milan, Italy.
  • Cervo L; Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via Balzaretti 9, 20133, Milan, Italy.
Psychopharmacology (Berl) ; 233(17): 3149-60, 2016 Sep.
Article in En | MEDLINE | ID: mdl-27370019
RATIONALE: Although brain-derived neurotrophic factor (BDNF) is part of a homeostatic pathway involved in the development of alcohol dependence, it is not clear whether this is also true after recreational ethanol consumption. OBJECTIVES: We examined BDNF expression and signaling in the cortico-striatal network immediately and 24 h after either a single intravenous (i.v.) ethanol operant self-administration session or the last of 14 sessions. METHODS: To compare contingent and non-contingent ethanol exposure, we incorporated the "yoked control-operant paradigm" in which rats actively taking ethanol (S-Et) were paired with two yoked controls receiving passive infusions of ethanol (Y-Et) or saline. RESULTS: A single ethanol exposure transiently reduced BDNF mRNA levels in the medial prefrontal cortex (mPFC) of Y-Et. Immediately after the last of 14 sessions, mRNA and mature BDNF protein levels (mBDNF) were reduced in the mPFC in both S-Et and Y-Et while mBDNF expression was raised in the nucleus accumbens (NAc), suggesting enhanced anterograde transport from the mPFC. Conversely, 24 h later mBDNF expression and signaling were raised in the mPFC and NAc of S-Et rats but reduced in the NAc of Y-Et rats, with concomitant reduction of downstream signaling pathways. CONCLUSIONS: Our findings indicate that recreational-like i.v. doses of ethanol promote early changes in neurotrophin expression, depending on the length and modality of administration, the brain region investigated, and the presence of the drug. A rapid intervention targeting the BDNF system might be useful to prevent escalation to alcohol abuse.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Central Nervous System Depressants / Prefrontal Cortex / Conditioning, Operant / Brain-Derived Neurotrophic Factor / Ethanol / Nucleus Accumbens Limits: Animals Language: En Journal: Psychopharmacology (Berl) Year: 2016 Document type: Article Affiliation country: Italy Country of publication: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Central Nervous System Depressants / Prefrontal Cortex / Conditioning, Operant / Brain-Derived Neurotrophic Factor / Ethanol / Nucleus Accumbens Limits: Animals Language: En Journal: Psychopharmacology (Berl) Year: 2016 Document type: Article Affiliation country: Italy Country of publication: Germany