Identification of Four Kinds of 2',3'-cNMPs in Escherichia coli and a Method for Their Preparation.
ACS Chem Biol
; 11(9): 2414-9, 2016 09 16.
Article
in En
| MEDLINE
| ID: mdl-27409357
ABSTRACT
Four kinds of 2',3'-cNMPs have been identified in animals and plants, and their physiological roles are also suggested. However, in prokaryotes, while 2',3'-cCMP and 2',3'-cUMP are reported, 2',3'-cGMP or 2',3'-cAMP have never been identified from bacteria or archaea. In addition, there has been no biological method to prepare these cyclic nucleotides. During the study of a novel gene LfliZ from deep-sea bacterium Pseudoalteromonas sp. SM9913, we found that the recombinant LfliZ oligomers contained endogenous substrates, which were then identified to be 2',3'-cCMP, 2',3'-cUMP, 2',3'-cGMP, and 2',3'-cAMP, showing the first evidence for the biological existence of four kinds of 2',3'-cNMPs in prokaryotes. In addition, we further developed a biological method to simultaneously prepare four kinds of 2',3'-cNMPs from Escherichia coli through expressing LfliZ.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nucleotides, Cyclic
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
ACS Chem Biol
Year:
2016
Document type:
Article