Ultraviolet scanning densitometry for detection, quantitation, and preparative elution of protein bands from unstained gels.
Anal Biochem
; 176(2): 400-5, 1989 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-2742129
ABSTRACT
Ultraviolet scanning of gel rods was used to identify and quantify protein bands in a nondestructive manner with good precision and sensitivity. This same technique, applied on a preparative scale, allowed quantitative protein elution, by reversed electrophoresis, from gel slices completely sealed in a dialysis bag. Protein recovery approached the theoretical yield (93.5 +/- 5%), with practically no interfering substances, and the entire preparative process (first electrophoresis, densitometric scanning, and reversed electrophoresis) could be performed in approximately 6 h. Its application to human growth hormone has shown no alteration in the biological activity of this protein.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteins
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Anal Biochem
Year:
1989
Document type:
Article
Affiliation country:
Brazil