An expert protocol for immunofluorescent detection of calcium channels in tsA-201 cells.

Koch, Peter; Herzig, Stefan; Matthes, Jan

Koch, Peter; Herzig, Stefan; Matthes, Jan.

Affiliation

Koch P; Department of Pharmacology, University Clinic Cologne, Gleuelerstrasse 24, D-50931 Cologne, Germany. Electronic address: peter.koch@uk-koeln.de.
Herzig S; Department of Pharmacology, University Clinic Cologne, Gleuelerstrasse 24, D-50931 Cologne, Germany. Electronic address: stefan.herzig@uni-koeln.de.
Matthes J; Department of Pharmacology, University Clinic Cologne, Gleuelerstrasse 24, D-50931 Cologne, Germany. Electronic address: jan.matthes@uk-koeln.de.

J Pharmacol Toxicol Methods ; 82: 20-25, 2016.

Article in En | MEDLINE | ID: mdl-27421665

Subject(s)

Calcium Channels, L-Type/genetics; Calcium Channels, L-Type/metabolism; Fluorescent Antibody Technique/methods; Single-Cell Analysis/methods; Cell Culture Techniques; Cell Line; Endoplasmic Reticulum/metabolism; Endosomes/metabolism; Golgi Apparatus/metabolism; Green Fluorescent Proteins/genetics; Humans; Immunohistochemistry; Protein Subunits; Transfection

Key words

Calcium channel; Immunocytochemistry; Membrane protein; Methods; Protein expression; tsA-201

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fluorescent Antibody Technique / Calcium Channels, L-Type / Single-Cell Analysis Type of study: Diagnostic_studies Limits: Humans Language: En Journal: J Pharmacol Toxicol Methods Journal subject: FARMACOLOGIA / TOXICOLOGIA Year: 2016 Document type: Article Country of publication: United States

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