Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags.
Biomarkers
; 22(2): 113-122, 2017 Mar.
Article
in En
| MEDLINE
| ID: mdl-27467182
ABSTRACT
CONTEXT AND OBJECTIVE:
Human hepatocellular carcinoma (HCC) is a severe malignant disease, and accurate and reliable diagnostic markers are still needed. This study was aimed for the discovery of novel marker candidates by quantitative proteomics. METHODS ANDRESULTS:
Proteomic differences between HCC and nontumorous liver tissue were studied by mass spectrometry. Among several significantly upregulated proteins, translocator protein 18 (TSPO) and Ras-related protein Rab-1A (RAB1A) were selected for verification by immunohistochemistry in an independent cohort. For RAB1A, a high accuracy for the discrimination of HCC and nontumorous liver tissue was observed.CONCLUSION:
RAB1A was verified to be a potent biomarker candidate for HCC.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carcinoma, Hepatocellular
/
Proteome
/
Tandem Mass Spectrometry
/
Liver Neoplasms
Limits:
Humans
Language:
En
Journal:
Biomarkers
Journal subject:
BIOQUIMICA
Year:
2017
Document type:
Article
Affiliation country:
Germany