Tomato yellow leaf curl virus: No evidence for replication in the insect vector Bemisia tabaci.
Sci Rep
; 6: 30942, 2016 08 01.
Article
in En
| MEDLINE
| ID: mdl-27476582
ABSTRACT
Begomovirus ssDNA plant virus (family Geminiviridae) replication within the Bemisia tabaci vector is controversial. Transovarial transmission, alteration to whitefly biology, or detection of viral transcripts in the vector are proposed as indirect evidence of replication of tomato yellow leaf curl virus (TYLCV). Recently, contrasting direct evidence has been reported regarding the capacity of TYLCV to replicate within individuals of B. tabaci based on quantitave PCR approaches. Time-course experiments to quantify complementary and virion sense viral nucleic acid accumulation within B. tabaci using a recently implemented two step qPCR procedure revealed that viral DNA quantities did not increase for time points up to 96 hours after acquisition of the virus. Our findings do not support a recent report claiming TYLCV replication in individuals of B. tabaci.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Diseases
/
Virion
/
Virus Replication
/
Begomovirus
/
Hemiptera
/
Insect Vectors
Limits:
Animals
Language:
En
Journal:
Sci Rep
Year:
2016
Document type:
Article
Affiliation country:
Spain