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Membrane Protein Production in Lactococcus lactis for Functional Studies.
Seigneurin-Berny, Daphne; King, Martin S; Sautron, Emiline; Moyet, Lucas; Catty, Patrice; André, François; Rolland, Norbert; Kunji, Edmund R S; Frelet-Barrand, Annie.
Affiliation
  • Seigneurin-Berny D; Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
  • King MS; Medical Research Council, Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 2XY, UK.
  • Sautron E; Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
  • Moyet L; Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
  • Catty P; Laboratoire de Chimie et Biologie des Métaux, CNRS (UMR-5249)/CEA/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
  • André F; Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris Saclay, Gif-sur-Yvette, France.
  • Rolland N; Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.
  • Kunji ER; Medical Research Council, Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 2XY, UK.
  • Frelet-Barrand A; Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris Saclay, Gif-sur-Yvette, France. annie.frelet-barrand@femto-st.fr.
Methods Mol Biol ; 1432: 79-101, 2016.
Article in En | MEDLINE | ID: mdl-27485331
Due to their unique properties, expression and study of membrane proteins in heterologous systems remains difficult. Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis, traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Engineering / Lactococcus lactis / Membrane Proteins Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Document type: Article Affiliation country: France Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Engineering / Lactococcus lactis / Membrane Proteins Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2016 Document type: Article Affiliation country: France Country of publication: United States