Curcumin inhibits osteoclastogenic potential in PBMCs from rheumatoid arthritis patients via the suppression of MAPK/RANK/c-Fos/NFATc1 signaling pathways.
Mol Med Rep
; 14(4): 3620-6, 2016 Oct.
Article
in En
| MEDLINE
| ID: mdl-27572279
The aim of the present study was to determine the effects of curcumin on the osteoclastogenic potential of peripheral blood mononuclear cells (PBMCs) obtained from patients with rheumatoid arthritis (RA), and to investigate the underlying molecular mechanisms. PBMCs from patients with RA (n=12) and healthy controls (n=10) were cultured to assess osteoclastogenic potential. The number of tartrateresistant acid phosphatasepositive osteoclasts differentiated from PBMCs isolated from patients with RA was significantly increased compared with that of the healthy controls. In addition, the osteoclast number in patients with RA was correlated with the clinical indicators, Sharp score (r=0.810; P=0.001) and lumbar Tscore (r=0.685; P=0.014). Furthermore, the resorption area was increased in the RA group compared with the healthy controls. The mRNA and protein expression levels in PBMCderived osteoclasts treated with curcumin were measured by reverse transcriptionquantitative polymerase chain reaction and western blotting, respectively. Curcumin inhibited the osteoclastogenic potential of PBMCs, potentially by suppressing activation of extracellular signalregulated kinases 1 and 2, p38 and cJun Nterminal kinase, and inhibiting receptor activator of nuclear factor κB (RANK), cFos and nuclear factor of activated T cells (NFATc1) expression. The results of the present study demonstrated that curcumin may inhibit the osteoclastogenic potential of PBMCs from patients with RA through the suppression of the mitogenactivated protein kinase/RANK/cFos/NFATc1 signaling pathways, and that curcumin may be a potential novel therapeutic agent for the treatment of bone deterioration in inflammatory diseases such as RA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoclasts
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Arthritis, Rheumatoid
/
Leukocytes, Mononuclear
/
Signal Transduction
/
Curcumin
/
Anti-Inflammatory Agents
Limits:
Humans
Language:
En
Journal:
Mol Med Rep
Year:
2016
Document type:
Article
Country of publication:
Greece