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Fluorescence-based measurement of cystine uptake through xCT shows requirement for ROS detoxification in activated lymphocytes.
Siska, Peter J; Kim, Bumki; Ji, Xiangming; Hoeksema, Megan D; Massion, Pierre P; Beckermann, Kathryn E; Wu, Jianli; Chi, Jen-Tsan; Hong, Jiyong; Rathmell, Jeffrey C.
Affiliation
  • Siska PJ; Department of Pathology, Microbiology, and Immunology, Vanderbilt Center for Immunobiology, Vanderbilt University Medical Center, Nashville, TN 37323, United States. Electronic address: peter.siska@vanderbilt.edu.
  • Kim B; Department of Chemistry, Duke University, Durham, NC 27708, United States.
  • Ji X; Division of Allergy, Pulmonary and Critical Care Medicine, Thoracic Oncology Center, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37323, United States.
  • Hoeksema MD; Division of Allergy, Pulmonary and Critical Care Medicine, Thoracic Oncology Center, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37323, United States.
  • Massion PP; Division of Allergy, Pulmonary and Critical Care Medicine, Thoracic Oncology Center, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37323, United States.
  • Beckermann KE; Division of Hematology/Oncology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37323, United States.
  • Wu J; Molecular Genetics and Microbiology, Duke University Medical Center Durham, NC 27710, United States.
  • Chi JT; Molecular Genetics and Microbiology, Duke University Medical Center Durham, NC 27710, United States.
  • Hong J; Department of Chemistry, Duke University, Durham, NC 27708, United States.
  • Rathmell JC; Department of Pathology, Microbiology, and Immunology, Vanderbilt Center for Immunobiology, Vanderbilt University Medical Center, Nashville, TN 37323, United States.
J Immunol Methods ; 438: 51-58, 2016 11.
Article in En | MEDLINE | ID: mdl-27594594
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: B-Lymphocytes / Lymphocyte Activation / T-Lymphocytes / Reactive Oxygen Species / Cystine Limits: Humans Language: En Journal: J Immunol Methods Year: 2016 Document type: Article Country of publication: Netherlands
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: B-Lymphocytes / Lymphocyte Activation / T-Lymphocytes / Reactive Oxygen Species / Cystine Limits: Humans Language: En Journal: J Immunol Methods Year: 2016 Document type: Article Country of publication: Netherlands