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Electroinduced Delivery of Hydrogel Nanoparticles in Colon 26 Cells, Visualized by Confocal Fluorescence System.
Atanasova, Severina; Nikolova, Biliana; Murayama, Shuhei; Stoyanova, Elena; Tsoneva, Iana; Zhelev, Zhivko; Aoki, Ichio; Bakalova, Rumiana.
Affiliation
  • Atanasova S; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
  • Nikolova B; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria nikolova@bio21.bas.bg bakalova@nirs.go.jp.
  • Murayama S; Department of Molecular Imaging and Theranostics, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, Japan.
  • Stoyanova E; Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia, Bulgaria.
  • Tsoneva I; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
  • Zhelev Z; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria Department of Physics, Biophysics and Radiology, Medical Faculty, Sofia University, Sofia, Bulgaria Department of Medical Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora, B
  • Aoki I; Department of Molecular Imaging and Theranostics, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, Japan.
  • Bakalova R; Department of Molecular Imaging and Theranostics, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, Japan Department of Physics, Biophysics and Radiology, Medical Faculty, Sofia University, Sofia, Bulgaria nikolova@bio21.bas.b
Anticancer Res ; 36(9): 4601-6, 2016 09.
Article in En | MEDLINE | ID: mdl-27630301
ABSTRACT

BACKGROUND:

Nano-scale drug delivery systems (nano-DDS) are under intense investigation. Nano-platforms are developed for specific administration of small molecules, drugs, genes, contrast agents [quantum dots (QDs)] both in vivo and in vitro. Electroporation is a biophysical phenomenon which consists of the application of external electrical pulses across the cell membrane. The aim of this study was to research electro-assisted Colon 26 cell line internalization of QDs and QD-loaded nano-hydrogels (polymersomes) visualized by confocal microscopy and their influence on cell viability. MATERIALS AND

METHODS:

The experiments were performed on the Colon 26 cancer cell line, using a confocal fluorescent imaging system and cell viability test.

RESULTS:

Electroporation facilitated the delivery of nanoparticles in vivo. We demonstrated increased voltage-dependent delivery of nanoparticles into cells after electrotreatment, without significant cell viability reduction.

CONCLUSION:

The delivery and retention of the polymersomes in vitro is a promising tool for future cancer treatment strategies and nanomedcine.
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Collection: 01-internacional Database: MEDLINE Main subject: Colonic Neoplasms / Microscopy, Confocal / Hydrogels / Nanoparticles Limits: Animals / Female / Humans Language: En Journal: Anticancer Res Year: 2016 Document type: Article Affiliation country: Bulgaria Publication country: GR / GRECIA / GREECE / GRÉCIA
Search on Google
Add to My VHL
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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Colonic Neoplasms / Microscopy, Confocal / Hydrogels / Nanoparticles Limits: Animals / Female / Humans Language: En Journal: Anticancer Res Year: 2016 Document type: Article Affiliation country: Bulgaria Publication country: GR / GRECIA / GREECE / GRÉCIA