Electroinduced Delivery of Hydrogel Nanoparticles in Colon 26 Cells, Visualized by Confocal Fluorescence System.
Anticancer Res
; 36(9): 4601-6, 2016 09.
Article
in En
| MEDLINE
| ID: mdl-27630301
ABSTRACT
BACKGROUND:
Nano-scale drug delivery systems (nano-DDS) are under intense investigation. Nano-platforms are developed for specific administration of small molecules, drugs, genes, contrast agents [quantum dots (QDs)] both in vivo and in vitro. Electroporation is a biophysical phenomenon which consists of the application of external electrical pulses across the cell membrane. The aim of this study was to research electro-assisted Colon 26 cell line internalization of QDs and QD-loaded nano-hydrogels (polymersomes) visualized by confocal microscopy and their influence on cell viability. MATERIALS ANDMETHODS:
The experiments were performed on the Colon 26 cancer cell line, using a confocal fluorescent imaging system and cell viability test.RESULTS:
Electroporation facilitated the delivery of nanoparticles in vivo. We demonstrated increased voltage-dependent delivery of nanoparticles into cells after electrotreatment, without significant cell viability reduction.CONCLUSION:
The delivery and retention of the polymersomes in vitro is a promising tool for future cancer treatment strategies and nanomedcine.Key words
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Colonic Neoplasms
/
Microscopy, Confocal
/
Hydrogels
/
Nanoparticles
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
Anticancer Res
Year:
2016
Document type:
Article
Affiliation country:
Bulgaria
Publication country:
GR
/
GRECIA
/
GREECE
/
GRÉCIA