[Combination of triptolide with sodium cantharidinate synergistically enhances apoptosis on hepatoma cell line 7721].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 41(9): 911-7, 2016 Sep 28.
Article
in Zh
| MEDLINE
| ID: mdl-27640789
ABSTRACT
OBJECTIVE:
To determine the combined cytotoxic effect and the molecular basis of triptolide and sodium cantharidinate on hepatoma cell line 7721.â©METHODS:
After treating the hepatoma cell line 7721 with triptolide(9, 18, or 36 µg/mL) and/or sodium cantharidinate (2, 5, or 10 µg/mL), cell viability assay and apoptosis were examined by MTT and flocytometry, respectively. The protein levels of caspase 3 and nuclear factor κB were analyzed by Western blot.â©RESULTS:
Viability of hepatoma cell line 7721 was inhibited by either the therapy of triptolide and/or sodium cantharidinate (P<0.05) in a time- and dose-dependent manner. The combined effects of both drugs were better than those of the single drug (P<0.05). The combined therapy down-regulated the expression of NF-κB p65 (P<0.05) while up-regulated the expression of caspase-3 (P<0.05).â©CONCLUSION:
Triptolide and sodium cantharidinate exert a synergistic toxic effect on hepatoma cell line 7721, which is related to increasing capase-3 activity and suppression of NF- κB.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenanthrenes
/
Cantharidin
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Diterpenes
Limits:
Humans
Language:
Zh
Journal:
Zhong Nan Da Xue Xue Bao Yi Xue Ban
Journal subject:
MEDICINA
Year:
2016
Document type:
Article
Affiliation country:
China