DNA methylation is not involved in specific down-regulation of HSD3B2, NR4A1 and RARB genes in androgen-secreting cells of human adrenal cortex.
Mol Cell Endocrinol
; 441: 46-54, 2017 02 05.
Article
in En
| MEDLINE
| ID: mdl-27670690
ABSTRACT
We hypothesized that DNA methylation is involved in human adrenal functional zonation. mRNAs expression and methylation pattern of RARB, NR4A1 and HSD3B2 genes in human adrenal tissues (HAT) and in pediatric virilizing adrenocortical tumors (VAT) were analyzed. For analysis of the results samples were divided into 3 age groups according to FeZ involution, pre and post-adrenarche ages. In all HAT, similar RARB mRNA was found including microdissected zona reticularis (ZR) and zona fasciculata, but HSD3B2 and NR4A1 mRNAs were lower in ZR (p < 0.05). NR4A1 and RARB promoters remained unmethylated in HAT and VAT. No adrenal zone-specific differences in NR4A1 methylation were observed. In summary, RARB was not associated with ZR-specific downregulation of HSD3B2 in postnatal human adrenocotical zonation. DNA methylation would not be involved in NR4A1 adrenocortical cell-type specific downregulation. Lack of CpG islands in HSD3B2 suggested that HSD3B2 ZR-specific downregulation would not be directly mediated by DNA methylation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Progesterone Reductase
/
Down-Regulation
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Adrenal Cortex
/
Receptors, Retinoic Acid
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DNA Methylation
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Nuclear Receptor Subfamily 4, Group A, Member 1
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Androgens
Limits:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Humans
/
Infant
Language:
En
Journal:
Mol Cell Endocrinol
Year:
2017
Document type:
Article
Publication country:
IE
/
IRELAND
/
IRLANDA