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Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering.
Liu, Qingshu; Shen, Qiyao; Bian, Xiaoying; Chen, Hanna; Fu, Jun; Wang, Hailong; Lei, Ping; Guo, Zhaohui; Chen, Wu; Li, Dingjun; Zhang, Youming.
Affiliation
  • Liu Q; College of Plant Protection, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
  • Shen Q; Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, 250100, People's Republic of China.
  • Bian X; Hunan Institute of Microbiology, Changsha, 410009, People's Republic of China.
  • Chen H; Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, 250100, People's Republic of China.
  • Fu J; Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, 250100, People's Republic of China.
  • Wang H; Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, 250100, People's Republic of China.
  • Lei P; Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, 250100, People's Republic of China.
  • Guo Z; Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, 250100, People's Republic of China.
  • Chen W; Hunan Institute of Microbiology, Changsha, 410009, People's Republic of China.
  • Li D; Hunan Institute of Microbiology, Changsha, 410009, People's Republic of China.
  • Zhang Y; College of Plant Protection, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
Sci Rep ; 6: 34623, 2016 Sep 30.
Article in En | MEDLINE | ID: mdl-27687863

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Sci Rep Year: 2016 Document type: Article Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Sci Rep Year: 2016 Document type: Article Country of publication: United kingdom