Effects of Berberine on NLRP3 and IL-1ß Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation.
Biomed Res Int
; 2016: 2503703, 2016.
Article
in En
| MEDLINE
| ID: mdl-27689075
ABSTRACT
Background. Urate crystals-induced inflammation is a critical factor during the initiation of gouty arthritis. Berberine is well known for its anti-inflammatory activity. However, the underlying effects of berberine on monosodium urate crystals-induced inflammation remain obscure. Objectives. This study is set to explore the protective effect and mechanism of berberine on monosodium urate crystals-induced inflammation in human monocytic THP-1 cells. Methods. The mRNA levels of NLRP3 and IL-1ß were measured by Real-Time PCR, and the protein levels of NLRP3 and IL-1ß were determined by ELISA, Western blot, and immunofluorescence. Results. The NLRP3 and IL-1ß expressions were significantly increased in model group compared to that in normal group (P < 0.05). Meanwhile, there was significant reduction in the expressions of NLRP3 and IL-1ß mRNA in groups 6.25 µM berberine and 25 µM berberine when compared with model group (P < 0.05). Conclusions. Therefore, berberine alleviates monosodium urate crystals-induced inflammation by downregulating NLRP3 and IL-1ß expressions. The regulatory effects of berberine may be related to the inactivation of NLRP3 inflammasome.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Prognostic_studies
Language:
En
Journal:
Biomed Res Int
Year:
2016
Document type:
Article