Improving anticancer drug development begins with cell culture: misinformation perpetrated by the misuse of cytotoxicity assays.

Eastman, Alan

Eastman, Alan.

Affiliation

Eastman A; Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.

Oncotarget ; 8(5): 8854-8866, 2017 Jan 31.

Article in En | MEDLINE | ID: mdl-27750219

Subject(s)

Antineoplastic Agents/pharmacology; Biological Assay; Drug Discovery/methods; Neoplasms/drug therapy; Toxicity Tests/methods; Animals; Antineoplastic Combined Chemotherapy Protocols/pharmacology; Cell Death/drug effects; Cell Line, Tumor; Cell Proliferation/drug effects; Cell Survival/drug effects; Dose-Response Relationship, Drug; Drug Synergism; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Humans; Neoplasms/genetics; Neoplasms/metabolism; Neoplasms/pathology; Reproducibility of Results; Time Factors

Key words

apoptosis; cell survival; cytotoxicity assays; synergy; target validation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Toxicity Tests / Drug Discovery / Neoplasms / Antineoplastic Agents Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Oncotarget Year: 2017 Document type: Article Affiliation country: United States Country of publication: United States

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