Label-free versus conventional cellular assays: Functional investigations on the human histamine H<sub>1</sub> receptor.

Lieb, S; Littmann, T; Plank, N; Felixberger, J; Tanaka, M; Schäfer, T; Krief, S; Elz, S; Friedland, K; Bernhardt, G; Wegener, J; Ozawa, T; Buschauer, A

Label-free versus conventional cellular assays: Functional investigations on the human histamine H₁ receptor.

Lieb, S; Littmann, T; Plank, N; Felixberger, J; Tanaka, M; Schäfer, T; Krief, S; Elz, S; Friedland, K; Bernhardt, G; Wegener, J; Ozawa, T; Buschauer, A.

Affiliation

Lieb S; Institute of Pharmacy, University of Regensburg, D-93040 Regensburg, Germany.
Littmann T; Institute of Pharmacy, University of Regensburg, D-93040 Regensburg, Germany.
Plank N; Institute of Pharmacy, University of Regensburg, D-93040 Regensburg, Germany.
Felixberger J; Institute of Pharmacy, University of Regensburg, D-93040 Regensburg, Germany.
Tanaka M; Department of Chemistry, School of Science, University of Tokyo, Tokyo, Japan.
Schäfer T; Department of Chemistry and Pharmacy, Molecular and Clinical Pharmacy, Friedrich-Alexander Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany.
Krief S; Bioprojet Biotech, 35762 Saint-Grégoire, France.
Elz S; Institute of Pharmacy, University of Regensburg, D-93040 Regensburg, Germany.
Friedland K; Department of Chemistry and Pharmacy, Molecular and Clinical Pharmacy, Friedrich-Alexander Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany.
Bernhardt G; Institute of Pharmacy, University of Regensburg, D-93040 Regensburg, Germany.
Wegener J; Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040 Regensburg, Germany.
Ozawa T; Department of Chemistry, School of Science, University of Tokyo, Tokyo, Japan.
Buschauer A; Institute of Pharmacy, University of Regensburg, D-93040 Regensburg, Germany. Electronic address: Armin.Buschauer@ur.de.

Pharmacol Res ; 114: 13-26, 2016 12.

Article in En | MEDLINE | ID: mdl-27751876

Subject(s)

Histamine Agonists/pharmacology; Histamine H1 Antagonists/pharmacology; Receptors, Histamine H1/metabolism; Calcium/metabolism; Drug Evaluation, Preclinical; Electric Impedance; GTP-Binding Proteins/metabolism; Genes, Reporter; HEK293 Cells; Histamine/pharmacology; Humans; Ligands; Radioligand Assay; Signal Transduction/drug effects; beta-Arrestins/metabolism

Key words

2-(3-Trifluoromethylphenyl)histamine (PubChem CID: 5310982); Betahistine dihydrochloride (CID: 68643); Calcium assay; Clozapine (PubChem CID: 2818); Cyproheptadine hydrochloride (PubChem CID: 13770); Diphenhydramine hydrochloride (PubChem CID: 8980); Dynamic mass redistribution; FR900359 (PubChem CID: 14101198); Fexofenadine hydrochloride (PubChem CID: 63002); Gallein (PubChem CID: 73685); Histamine H(1) receptor; Histamine dihydrochloride (PubChem CID: 5818); Histaprodifen (PubChem CID: 10447834); Impedimetry; Levocetirizine dihydrochloride (PubChem CID: 9955977); Maprotiline hydrochloride (PubChem CID: 71478); Mepyramine maleate (PubChem CID: 5284451); Mirtazapine (PubChem CID: 4205); Reporter gene assay; [(3)H]mepyramine (PubChem CID: 656400); beta-Arrestin recruitment

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Receptors, Histamine H1 / Histamine Agonists / Histamine H1 Antagonists Limits: Humans Language: En Journal: Pharmacol Res Journal subject: FARMACOLOGIA Year: 2016 Document type: Article Affiliation country: Germany Country of publication: Netherlands

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