Measuring Telomerase Activity in Senescent Human T Cells Upon Genetic Modification.
Methods Mol Biol
; 1514: 119-126, 2017.
Article
in En
| MEDLINE
| ID: mdl-27787797
ABSTRACT
Telomerase, a RNA-dependent DNA polymerase that adds telomeric DNA at the 3' ends of eukaryotic chromosomes, is essential for the lifelong preservation of the proliferative potential of antigen specific T lymphocytes. However, senescent T cells that have low telomerase activity, short telomeres and lack of replicative capacity accumulate in old humans, patients with chronic viral infections and cancer. The mechanisms inhibiting telomerase in these cells are poorly understood. Here I describe a strategy that was successfully applied to identify pathways causing telomerase dysfunction in primary human senescent T lymphocytes. Such strategy couples lentiviral vector-based gene manipulations to functional and signaling readouts directly ex vivo, in humans.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
T-Lymphocytes
/
Cellular Senescence
/
Telomerase
/
Molecular Biology
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
United kingdom