Saponin-based adjuvants induce cross-presentation in dendritic cells by intracellular lipid body formation.
Nat Commun
; 7: 13324, 2016 11 07.
Article
in En
| MEDLINE
| ID: mdl-27819292
ABSTRACT
Saponin-based adjuvants (SBAs) are being used in animal and human (cancer) vaccines, as they induce protective cellular immunity. Their adjuvant potency is a factor of inflammasome activation and enhanced antigen cross-presentation by dendritic cells (DCs), but how antigen cross-presentation is induced is not clear. Here we show that SBAs uniquely induce intracellular lipid bodies (LBs) in the CD11b+ DC subset in vitro and in vivo. Using genetic and pharmacological interference in models for vaccination and in situ tumour ablation, we demonstrate that LB induction is causally related to the saponin-dependent increase in cross-presentation and T-cell activation. These findings link adjuvant activity to LB formation, aid the application of SBAs as a cancer vaccine component, and will stimulate development of new adjuvants enhancing T-cell-mediated immunity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saponins
/
Skin Neoplasms
/
Dendritic Cells
/
Melanoma, Experimental
/
Cancer Vaccines
/
Cross-Priming
Type of study:
Prognostic_studies
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
Nat Commun
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2016
Document type:
Article
Affiliation country:
Netherlands