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The Landscape of Viral Expression Reveals Clinically Relevant Viruses with Potential Capability of Promoting Malignancy in Lower-Grade Glioma.
Wang, Zheng; Hao, Yajing; Zhang, Chuanbao; Wang, Zhiliang; Liu, Xing; Li, Guanzhang; Sun, Lihua; Liang, Jingshan; Luo, Jianjun; Zhou, Dabiao; Chen, Runsheng; Jiang, Tao.
Affiliation
  • Wang Z; Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
  • Hao Y; Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
  • Zhang C; Chinese Glioma Genome Atlas Network, Beijing, China.
  • Wang Z; Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Liu X; Beijing Key Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Li G; University of Chinese Academy of Sciences, Beijing, China.
  • Sun L; Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
  • Liang J; Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
  • Luo J; Chinese Glioma Genome Atlas Network, Beijing, China.
  • Zhou D; Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
  • Chen R; Chinese Glioma Genome Atlas Network, Beijing, China.
  • Jiang T; Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Clin Cancer Res ; 23(9): 2177-2185, 2017 May 01.
Article in En | MEDLINE | ID: mdl-27864420
ABSTRACT

Purpose:

RNA sequencing (RNA-seq) has recently proved to be effective for revealing novel virus-tumor associations. To get a thorough investigation of virus-glioma associations, we screened viruses in gliomas with RNA-seq data from the Chinese Glioma Genome Atlas project.Experimental

Design:

In total, 325 samples were enrolled into this study. Reads that failed to map to the human genome were aligned to viral genomes and screened for potential virus-derived transcripts. For quantification, VPKM was calculated according to mapped reads weighted by genome sizes and sequencing depth.

Results:

We observed that viruses tended to concertedly express in a certain subgroup of patients. Survival analysis revealed that individuals who were infected with Simian virus 40 (SV40) or woolly monkey sarcoma virus (WMSV) had a significantly shorter overall survival than those uninfected. A multivariate Cox proportional hazards model, taking clinical and molecular factors into account, was applied to assess the prognostic value of SV40 and WMSV. Both SV40 and WMSV were independent prognostic factors for predicting patient's survival in lower-grade gliomas. Subsequent gene analysis demonstrated that SV40 was correlated with regulation of transcription, whereas WMSV was correlated with cell-cycle phase, which indicated frequent proliferation of tumor cells.

Conclusions:

RNA-seq was sufficient to identify virus infection in glioma samples. SV40 and WMSV were identified to be prognostic markers for patients with lower-grade gliomas and showed potential values for targeting therapy. Clin Cancer Res; 23(9); 2177-85. ©2016 AACR.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sarcoma Virus, Woolly Monkey / Simian virus 40 / Endogenous Retroviruses / Glioma Type of study: Prognostic_studies Limits: Female / Humans / Male Language: En Journal: Clin Cancer Res Journal subject: NEOPLASIAS Year: 2017 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sarcoma Virus, Woolly Monkey / Simian virus 40 / Endogenous Retroviruses / Glioma Type of study: Prognostic_studies Limits: Female / Humans / Male Language: En Journal: Clin Cancer Res Journal subject: NEOPLASIAS Year: 2017 Document type: Article Affiliation country: China