Identifying Bacterial Immune Evasion Proteins Using Phage Display.
Methods Mol Biol
; 1535: 43-61, 2017.
Article
in En
| MEDLINE
| ID: mdl-27914072
ABSTRACT
Methods aimed at identification of immune evasion proteins are mainly rely on in silico prediction of sequence, structural homology to known evasion proteins or use a proteomics driven approach. Although proven successful these methods are limited by a low efficiency and or lack of functional identification. Here we describe a high-throughput genomic strategy to functionally identify bacterial immune evasion proteins using phage display technology. Genomic bacterial DNA is randomly fragmented and ligated into a phage display vector that is used to create a phage display library expressing bacterial secreted and membrane bound proteins. This library is used to select displayed bacterial secretome proteins that interact with host immune components.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacteria
/
Bacterial Proteins
/
Immune Evasion
/
Cell Surface Display Techniques
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
Netherlands