[Mutations in genes affecting fertility of men - current routine laboratory genetic diagnostics and searching for more DNA segments and genes influencing spermatogenesis]. / Mutace v genech ovlivnujících plodnost muzu - soucasná rutinní laboratorní genetická diagnostika a hledání dalsích úseku DNA a genu, ovlivnujících spermatogenezi.
Ceska Gynekol
; 81(6): 437-443, 2016.
Article
in Cs
| MEDLINE
| ID: mdl-27918162
ABSTRACT
OBJECTIVE:
To present the results of molecular genetics analysis in men with reproductive disorders focusing on the DNA segments and genes which affect spermatogenesis.DESIGN:
Original article.SETTING:
Institute of Biology and Medical Genetics of the First Faculty of Medicine and General Teaching Hospital, Prague.METHODS:
One hundred and twenty-three patients identified with a fertility disorder were screened for mutations of the CFTR gene. In all patients were performed cytogenic analysis and assessment of Y-chromosome microdeletions. In 107 patients where the fertility was not detected by routine examination we performed an analysis for X-chromosome microdeletions (CNV64, CNV67, CNV69) and in certain genes necessary for normal spermatogenesis (AGFG1, CAPZA3, CNTROB, HOOK1, GOPC, SPATA16).RESULTS:
Our results did not reveal any negative efffects of X-chromosome microdeletion on spermatogenesis. Analysis of six genes showed in two patients in gene SPATA16 a homozygotic haplotype [1526C>T + 1577T>C] which can be most probably responsible for the fertility in two examined patients.CONCLUSION:
According to our results we do not recommend introduction of X-chromosome microdeletions assays in areas CNV64 , CNV67 and CNV69 into routine diagnostic. Regarding the selected genes affecting spermatogenesis, our results showed that homozygotic haplotype [ 1526C>T + 1577T>C] in SPATA16 gene is very likely responsible for infertility in two of our patients. The above mentioned haplotype deserves attention in the investigation of male infertility.Key words
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatogenesis
/
Infertility, Male
Type of study:
Diagnostic_studies
Limits:
Humans
/
Male
Language:
Cs
Journal:
Ceska Gynekol
Journal subject:
GINECOLOGIA
Year:
2016
Document type:
Article