In vitro anti-MRSA activity of Couroupita guianensis extract and its component Tryptanthrin.
Nat Prod Res
; 31(17): 2077-2080, 2017 Sep.
Article
in En
| MEDLINE
| ID: mdl-28013553
ABSTRACT
Couroupita guianensis is known in Brazil as 'Abricó-de-Macaco' and it has some attributes such as antihypertensive, analgesic and anti-inflammatory activities. This study evaluated the antimicrobial activity of ethanolic extract and fractions of C. guianensis flowers and isolation of bioactive component. These extracts and fractions were subjected to agar diffusion, MIC, TLC and bioautography to bacteria, filamentous fungi and yeasts. Among the fractions of EtOH extract, the DCM fraction was the most active, particularly against Methicillin-resistant Staphylococcus aureus (MRSA) with MIC of 156 µg/mL. The active compound in this fraction was identified as Tryptanthrin, which showed promising antibacterial activity for MRSA showing MIC of 62.5 µg/mL. Ultrastructural analysis of MRSA incubated in the presence of Tryptanthrin by transmission electron microscope showed significant alterations in the cellular structure. Cytotoxicity tests demonstrated that DCM fraction and Tryptanthrin showed low toxicity, which makes it a promising candidate for alternative therapies to control and combat diseases.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Quinazolines
/
Lecythidaceae
/
Methicillin-Resistant Staphylococcus aureus
/
Anti-Bacterial Agents
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Nat Prod Res
Year:
2017
Document type:
Article
Affiliation country:
Brazil