Glyoxal and methylglyoxal determination in urine by surfactant-assisted dispersive liquid-liquid microextraction and LC.
Bioanalysis
; 9(4): 369-379, 2017 Feb.
Article
in En
| MEDLINE
| ID: mdl-28102703
ABSTRACT
AIM:
Two important markers of oxidative stress, glyoxal and methylglyoxal, are preconcentrated from human urine by surfactant-assisted dispersive liquid-liquid microextraction and separated by LC-fluorescence. METHODS/RESULTS:
Derivatization was carried out overnight with 0.8 mM 2,3-diaminonaphthalene at 4°C. For surfactant-assisted dispersive liquid-liquid microextraction, 500 µl buffer solution (pH 10.5) and 25 µl 0.03 M Triton X-114 were added to 2.5 ml of the sample and the mixture was made up to 10 ml before the rapid injection of 75 µl 1-undecanol (extractant solvent) and 0.5 ml ethanol (dispersant solvent).CONCLUSION:
The method can be applied to analyze glyoxal and methylglyoxal in urine with LOD of 13 and 16 ng/l, respectively, and recoveries in the 88-103% range.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyruvaldehyde
/
Surface-Active Agents
/
Chromatography, Liquid
/
Urinalysis
/
Liquid Phase Microextraction
/
Glyoxal
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Bioanalysis
Year:
2017
Document type:
Article
Affiliation country:
Spain