A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity.
Methods Mol Biol
; 1564: 49-61, 2017.
Article
in En
| MEDLINE
| ID: mdl-28124246
ABSTRACT
Pathway cross-communication cannot be simply tackled by studying isolated signaling systems. Yet understanding how signal transduction pathways attenuate or reinforce each other in vivo is a challenging task. In plants, biosynthesis and signaling of brassinosteroids (BRs) finely regulate growth and defense programs through a complex array of mechanistic and physiological interactions. Conversely, induction of defenses also impacts on the BR biosynthesis at the transcriptional level. In this chapter, we present an experimental framework to study the physiological connection between BR-controlled growth and defenses. We focus on the signaling pathways regulated by the two archetypal cell surface receptors, BRASSINOSTEROID INSENSITIVE1 (BRI1) and FLAGELLIN-SENSITIVE2 (FLS2), to illustrate the signaling nexus of BRs and plant immunity. In Arabidopsis thaliana, these pathways provide one of the very few systems in which the tools and mechanistic details exist to study cross talk at the molecular level.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Growth Regulators
/
Protein Kinases
/
Steroids, Heterocyclic
/
Arabidopsis
/
Gene Expression Regulation, Plant
/
Arabidopsis Proteins
/
Plant Immunity
/
Brassinosteroids
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
China