Repair of bone defects with prefabricated vascularized bone grafts and double-labeled bone marrow-derived mesenchymal stem cells in a rat model.
Sci Rep
; 7: 39431, 2017 02 02.
Article
in En
| MEDLINE
| ID: mdl-28150691
ABSTRACT
This study aims to investigate the repair of bone defects with prefabricated vascularized bone grafts and double-labeled bone marrow-derived mesenchymal stem cells (BMSCs) in a rat model. BMSCs were separated from rat bone marrow. LTR-CMVpro-RFP and LTR-CMVpro-GFP were transfected into the BMSCs for in vitro and in vivo tracking. BMSCs-RFP and BMSCs-GFP were induced into endothelial progenitor cells (EPCs) and osteoblasts (OBs). Rats were divided into five groups Group A in vitro prefabrication with EPCs-RFP + in vivo prefabrication with arteriovenous vascular bundle + secondary OBs-GFP implantation; Group B in vitro prefabrication with EPCs-RFP + secondary OBs-GFP implantation; Group C in vivo prefabrication with arteriovenous vascular bundle + secondary OBs-GFP implantation; Group D implantation of EPCs-RFP + implantation of with arteriovenous vascular bundle + simultaneous OBs-GFP implantation; Group E demineralized bone matrix (DBM) grafts (blank control). Among five groups, Group A had the fastest bone regeneration and repair, and the regenerated bone highly resembled normal bone tissues; Group D also had fast bone repair, but the repair was slightly slower than Group A. Therefore, in vitro prefabrication with EPCs-RFP plus in vivo prefabrication with arteriovenous vascular bundle and secondary OBs-GFP implantation could be the best treatment for bone defect.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bone Diseases
/
Bone Transplantation
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Cell Transplantation
/
Mesenchymal Stem Cells
Limits:
Animals
Language:
En
Journal:
Sci Rep
Year:
2017
Document type:
Article