HTLV-1 Tax activates HIV-1 transcription in latency models.
Virology
; 504: 45-51, 2017 04.
Article
in En
| MEDLINE
| ID: mdl-28152383
ABSTRACT
HIV-1 latency is a major obstacle to HIV-1 eradication. Coinfection with HTLV-1 has been associated with faster progression to AIDS. HTLV-1 encodes the transactivator Tax which can activate both HTLV-1 and HIV-1 transcription. Here, we demonstrate that Tax activates HIV transcription in latent CD4+ T cells. Tax promotes the activation of P-TEFb, releasing CDK9 and Cyclin T1 from inactive forms, promoting transcription elongation and reactivation of latent HIV-1. Tax mutants lacking interaction with the HIV-1-LTR promoter were not able to activate P-TEFb, with no subsequent activation of latent HIV. In HIV-infected primary resting CD4+ T cells, Tax-1 reactivated HIV-1 transcription up to five fold, confirming these findings in an ex vivo latency model. Finally, our results confirms that HTLV-1/Tax hijacks cellular partners, promoting HIV-1 transcription, and this interaction should be further investigated in HIV-1 latency studies in patients with HIV/HTLV-1 co-infection.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
Human T-lymphotropic virus 1
/
CD4-Positive T-Lymphocytes
/
Transcriptional Activation
/
Gene Products, tax
/
HIV-1
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Virology
Year:
2017
Document type:
Article
Affiliation country:
Brazil