Integrative analyses of transcriptome sequencing identify novel functional lncRNAs in esophageal squamous cell carcinoma.

Li, C-Q; Huang, G-W; Wu, Z-Y; Xu, Y-J; Li, X-C; Xue, Y-J; Zhu, Y; Zhao, J-M; Li, M; Zhang, J; Wu, J-Y; Lei, F; Wang, Q-Y; Li, S; Zheng, C-P; Ai, B; Tang, Z-D; Feng, C-C; Liao, L-D; Wang, S-H; Shen, J-H; Liu, Y-J; Bai, X-F; He, J-Z; Cao, H-H; Wu, B-L; Wang, M-R; Lin, D-C; Koeffler, H P; Wang, L-D; Li, X; Li, E-M; Xu, L-Y

Li, C-Q; Huang, G-W; Wu, Z-Y; Xu, Y-J; Li, X-C; Xue, Y-J; Zhu, Y; Zhao, J-M; Li, M; Zhang, J; Wu, J-Y; Lei, F; Wang, Q-Y; Li, S; Zheng, C-P; Ai, B; Tang, Z-D; Feng, C-C; Liao, L-D; Wang, S-H; Shen, J-H; Liu, Y-J; Bai, X-F; He, J-Z; Cao, H-H; Wu, B-L; Wang, M-R; Lin, D-C; Koeffler, H P; Wang, L-D; Li, X; Li, E-M; Xu, L-Y.

Affiliation

Li CQ; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Huang GW; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Wu ZY; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Xu YJ; Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, China.
Li XC; College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China.
Xue YJ; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Zhu Y; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Zhao JM; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Li M; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Zhang J; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Wu JY; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Lei F; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Wang QY; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Li S; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Zheng CP; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Ai B; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Tang ZD; College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China.
Feng CC; Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, China.
Liao LD; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Wang SH; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Shen JH; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Liu YJ; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Bai XF; Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, China.
He JZ; Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, China.
Cao HH; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Wu BL; School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing, China.
Wang MR; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Lin DC; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Koeffler HP; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
Wang LD; Cancer Institute/Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
Li X; Division of Hematology/Oncology, Cedars-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, CA, USA.
Li EM; Division of Hematology/Oncology, Cedars-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, CA, USA.
Xu LY; Cancer Science Institute of Singapore, National University of Singapore, Singapore, Singapore.

Oncogenesis ; 6(2): e297, 2017 Feb 13.

Article in En | MEDLINE | ID: mdl-28194033

ABSTRACT

ABSTRACT

Long non-coding RNAs (lncRNAs) have a critical role in cancer initiation and progression, and thus may mediate oncogenic or tumor suppressing effects, as well as be a new class of cancer therapeutic targets. We performed high-throughput sequencing of RNA (RNA-seq) to investigate the expression level of lncRNAs and protein-coding genes in 30 esophageal samples, comprised of 15 esophageal squamous cell carcinoma (ESCC) samples and their 15 paired non-tumor tissues. We further developed an integrative bioinformatics method, denoted URW-LPE, to identify key functional lncRNAs that regulate expression of downstream protein-coding genes in ESCC. A number of known onco-lncRNA and many putative novel ones were effectively identified by URW-LPE. Importantly, we identified lncRNA625 as a novel regulator of ESCC cell proliferation, invasion and migration. ESCC patients with high lncRNA625 expression had significantly shorter survival time than those with low expression. LncRNA625 also showed specific prognostic value for patients with metastatic ESCC. Finally, we identified E1A-binding protein p300 (EP300) as a downstream executor of lncRNA625-induced transcriptional responses. These findings establish a catalog of novel cancer-associated functional lncRNAs, which will promote our understanding of lncRNA-mediated regulation in this malignancy.

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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Prognostic_studies Language: En Journal: Oncogenesis Year: 2017 Document type: Article Affiliation country: China

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