Small Molecule Mixture Analysis by Heteronuclear NMR under Spin Diffusion Conditions in Viscous DMSO-Water Solvent.
Chemistry
; 23(20): 4923-4928, 2017 Apr 06.
Article
in En
| MEDLINE
| ID: mdl-28195380
ABSTRACT
Spin diffusion in NMR occurs for small- and medium-sized molecules when their tumbling rate reduces in solution so that magnetization exchange by longitudinal cross relaxation becomes highly efficient. Composite DMSO-water viscous solvents were used for the first time to access the individual NMR spectra of a mixture components in spin diffusion conditions. The easy handling and high dissolution power of [D6 ]DMSO/H2 O offers a wide range of potential applications for polar and moderately apolar mixture analysis. In addition to 2D 1 H-1 H NOESY and 1 H-13 C HSQC-NOESY, 1 H-15 N HSQC-NOESY, 1D and 2D 1 H-19 F heteronuclear NOESY (HOESY) experiments were set up to offer new ways to individualize molecules within a mixture. This article reports the analysis of a polar mixture of four dipeptides dissolved in [D6 ]DMSO/H2 O (73 v/v) and that of a medium-polarity fluorinated dinucleotide dissolved in [D6 ]DMSO/H2 O (82 v/v) by means of spin diffusion in NOESY, HOESY, and HSQC-NOESY experiments.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Chemistry
Journal subject:
QUIMICA
Year:
2017
Document type:
Article
Affiliation country:
France