Mertk gene expression and photoreceptor outer segment phagocytosis by cultured rat bone marrow mesenchymal stem cells.
Mol Vis
; 23: 8-19, 2017.
Article
in En
| MEDLINE
| ID: mdl-28210098
ABSTRACT
BACKGROUND:
Bone marrow mesenchymal stem cells (BM-MSCs) are multipotential stem cells that have been used for a broad spectrum of indications. Several investigations have used BM-MSCs to promote photoreceptor survival and suggested that BM-MSCs are a potential source of cell replacement therapy for some forms of retinal degeneration.PURPOSE:
To investigate the expression of the MER proto-oncogene, tyrosine kinase (Mertk), involved in the disruption of RPE phagocytosis and the onset of autosomal recessive retinitis pigmentosa in rat BM-MSCs and to compare phagocytosis of the photoreceptor outer segment (POS) by BM-MSCs and RPE cells in vitro.METHODS:
MSCs were isolated from the bone marrow of Brown Norway rats. Reverse transcription-PCR (RT-PCR) and western blot analyses were used to examine the expression of Mertk. The phagocytized POS was detected with double fluorescent labeling, transmission electron microscopy, and scanning electron microscopy.RESULTS:
Mertk expression did not differ among the first three passages of BM-MSCs. Mertk gene expression was greater in the BM-MSCs than the RPE cells. Mertk protein expression in the BM-MSCs was similar to that in the RPE cells in the primary passage and was greater than that in the RPE cells in the other two passages. BM-MSCs at the first three passages phagocytized the POS more strongly than the RPE cells. The process of BM-MSC phagocytosis was similar to that of the RPE cells.CONCLUSIONS:
BM-MSCs may be an effective cell source for treating retinal degeneration in terms of phagocytosis of the POS.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phagocytosis
/
Bone Marrow Cells
/
Gene Expression Regulation
/
Retinal Photoreceptor Cell Outer Segment
/
Mesenchymal Stem Cells
/
C-Mer Tyrosine Kinase
Limits:
Animals
Language:
En
Journal:
Mol Vis
Journal subject:
BIOLOGIA MOLECULAR
/
OFTALMOLOGIA
Year:
2017
Document type:
Article
Affiliation country:
China