Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.
Stem Cell Reports
; 8(3): 519-528, 2017 03 14.
Article
in En
| MEDLINE
| ID: mdl-28216146
ABSTRACT
In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Psychotic Disorders
/
Genetic Markers
/
Chromosomes, Human
/
Induced Pluripotent Stem Cells
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Stem Cell Reports
Year:
2017
Document type:
Article
Affiliation country:
United States