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Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.
Tcw, Julia; Carvalho, Claudia M B; Yuan, Bo; Gu, Shen; Altheimer, Alyssa N; McCarthy, Shane; Malhotra, Dheeraj; Sebat, Jonathan; Siegel, Arthur J; Rudolph, Uwe; Lupski, James R; Levy, Deborah L; Brennand, Kristen J.
Affiliation
  • Tcw J; Departments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA; Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA.
  • Carvalho CMB; Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA. Electronic address: cfonseca@bcm.edu.
  • Yuan B; Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
  • Gu S; Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
  • Altheimer AN; Departments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA; Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA.
  • McCarthy S; Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
  • Malhotra D; F. Hoffmann-La Roche Ltd, 4070 Basel, Switzerland.
  • Sebat J; Departments of Psychiatry, Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
  • Siegel AJ; Department of Internal Medicine, McLean Hospital, Belmont, MA 02478, USA; Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
  • Rudolph U; Laboratory of Genetic Neuropharmacology, McLean Hospital, Belmont, MA 02478, USA; Department of Psychiatry, Harvard Medical School, Boston, MA 02115, USA.
  • Lupski JR; Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA; Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA; Texas Children's Hospital, Houston, TX 77030, USA; Department of Pediatrics, Baylor College of Medicin
  • Levy DL; Department of Psychiatry, Harvard Medical School, Boston, MA 02115, USA; Psychology Research Laboratory, McLean Hospital, Belmont, MA 02478, USA. Electronic address: dlevy@mclean.harvard.edu.
  • Brennand KJ; Departments of Neuroscience, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA; Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA; Department of Psychiatry, Icahn School of Medicine at Mount Sinai, 1425
Stem Cell Reports ; 8(3): 519-528, 2017 03 14.
Article in En | MEDLINE | ID: mdl-28216146
ABSTRACT
In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Psychotic Disorders / Genetic Markers / Chromosomes, Human / Induced Pluripotent Stem Cells Type of study: Prognostic_studies Limits: Humans Language: En Journal: Stem Cell Reports Year: 2017 Document type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Psychotic Disorders / Genetic Markers / Chromosomes, Human / Induced Pluripotent Stem Cells Type of study: Prognostic_studies Limits: Humans Language: En Journal: Stem Cell Reports Year: 2017 Document type: Article Affiliation country: United States