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[Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation].
Wang, Tao; Yan, Rui-Qiao; Cao, Jun; Cao, Ling-Ling; Zhang, Xuan-Pu; Li, Xing-Nuan; Wu, Ping; Zhou, Xiao-Ou; Wu, Jian-Fang; Xu, Xiao-Yuan.
Affiliation
  • Wang T; 1Key Laboratory of Jiangxi Province for Systems Biomedicine, Jiujiang 332000, China; 2Department of Pathogenic Biology, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, China. E-mail: comwangtaocom@163.com.
Nan Fang Yi Ke Da Xue Xue Bao ; 37(2): 199-203, 2016 Feb 20.
Article in Zh | MEDLINE | ID: mdl-28219863
OBJECTIVE: To screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation. METHODS: Cultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing (RNA-seq) techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest. The databases including TargetScan, PicTar and miRanda were used to predict the target genes of the differentially expressed miRNA. RESULTS: The expression of miR-140-5p was down-regulated and leukemia inhibitory factor receptor (LIFR) expression increased progressively during adipogenic differentiation of hMSCs, showing a negative correlation between them. Target gene prediction using the 3 databases identified LIFR as the target gene of miR-140-5p. CONCLUSION: miRNA-140-5p may play an important role by regulating its target gene LIFR during adipogenic differentiation of hMSCs.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / MicroRNAs / Adipogenesis / Mesenchymal Stem Cells Type of study: Prognostic_studies / Risk_factors_studies Limits: Humans Language: Zh Journal: Nan Fang Yi Ke Da Xue Xue Bao Year: 2016 Document type: Article Country of publication: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / MicroRNAs / Adipogenesis / Mesenchymal Stem Cells Type of study: Prognostic_studies / Risk_factors_studies Limits: Humans Language: Zh Journal: Nan Fang Yi Ke Da Xue Xue Bao Year: 2016 Document type: Article Country of publication: China