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Neisseria gonorrhoeae Sequence Typing for Antimicrobial Resistance, a Novel Antimicrobial Resistance Multilocus Typing Scheme for Tracking Global Dissemination of N. gonorrhoeae Strains.
Demczuk, W; Sidhu, S; Unemo, M; Whiley, D M; Allen, V G; Dillon, J R; Cole, M; Seah, C; Trembizki, E; Trees, D L; Kersh, E N; Abrams, A J; de Vries, H J C; van Dam, A P; Medina, I; Bharat, A; Mulvey, M R; Van Domselaar, G; Martin, I.
Affiliation
  • Demczuk W; Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, MB, Canada.
  • Sidhu S; Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, MB, Canada.
  • Unemo M; WHO Collaborating Centre for Gonorrhoea and Other STIs, Örebro University Hospital, Örebro, Sweden.
  • Whiley DM; The University of Queensland, Centre for Clinical Research, Brisbane, Australia.
  • Allen VG; Public Health Ontario Laboratories, Toronto, Ontario, Canada.
  • Dillon JR; Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
  • Cole M; Public Health England, London, United Kingdom.
  • Seah C; Public Health Ontario Laboratories, Toronto, Ontario, Canada.
  • Trembizki E; The University of Queensland, Centre for Clinical Research, Brisbane, Australia.
  • Trees DL; Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Kersh EN; Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Abrams AJ; Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • de Vries HJC; STI Outpatient Clinic, Department of Infectious Diseases, Public Health Service Amsterdam, Amsterdam, the Netherlands.
  • van Dam AP; Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Medina I; Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
  • Bharat A; Public Health Laboratory, Public Health Service Amsterdam, Amsterdam, the Netherlands.
  • Mulvey MR; Department of Medical Microbiology, OLVG General Hospital, Amsterdam, the Netherlands.
  • Van Domselaar G; Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, MB, Canada.
  • Martin I; Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, MB, Canada.
J Clin Microbiol ; 55(5): 1454-1468, 2017 05.
Article in En | MEDLINE | ID: mdl-28228492
A curated Web-based user-friendly sequence typing tool based on antimicrobial resistance determinants in Neisseria gonorrhoeae was developed and is publicly accessible (https://ngstar.canada.ca). The N. gonorrhoeae Sequence Typing for Antimicrobial Resistance (NG-STAR) molecular typing scheme uses the DNA sequences of 7 genes (penA, mtrR, porB, ponA, gyrA, parC, and 23S rRNA) associated with resistance to ß-lactam antimicrobials, macrolides, or fluoroquinolones. NG-STAR uses the entire penA sequence, combining the historical nomenclature for penA types I to XXXVIII with novel nucleotide sequence designations; the full mtrR sequence and a portion of its promoter region; portions of ponA, porB, gyrA, and parC; and 23S rRNA sequences. NG-STAR grouped 768 isolates into 139 sequence types (STs) (n = 660) consisting of 29 clonal complexes (CCs) having a maximum of a single-locus variation, and 76 NG-STAR STs (n = 109) were identified as unrelated singletons. NG-STAR had a high Simpson's diversity index value of 96.5% (95% confidence interval [CI] = 0.959 to 0.969). The most common STs were NG-STAR ST-90 (n = 100; 13.0%), ST-42 and ST-91 (n = 45; 5.9%), ST-64 (n = 44; 5.72%), and ST-139 (n = 42; 5.5%). Decreased susceptibility to azithromycin was associated with NG-STAR ST-58, ST-61, ST-64, ST-79, ST-91, and ST-139 (n = 156; 92.3%); decreased susceptibility to cephalosporins was associated with NG-STAR ST-90, ST-91, and ST-97 (n = 162; 94.2%); and ciprofloxacin resistance was associated with NG-STAR ST-26, ST-90, ST-91, ST-97, ST-150, and ST-158 (n = 196; 98.0%). All isolates of NG-STAR ST-42, ST-43, ST-63, ST-81, and ST-160 (n = 106) were susceptible to all four antimicrobials. The standardization of nomenclature associated with antimicrobial resistance determinants through an internationally available database will facilitate the monitoring of the global dissemination of antimicrobial-resistant N. gonorrhoeae strains.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Drug Resistance, Multiple, Bacterial / Multilocus Sequence Typing / Anti-Bacterial Agents / Neisseria gonorrhoeae Type of study: Prognostic_studies Limits: Humans Language: En Journal: J Clin Microbiol Year: 2017 Document type: Article Affiliation country: Canada Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Drug Resistance, Multiple, Bacterial / Multilocus Sequence Typing / Anti-Bacterial Agents / Neisseria gonorrhoeae Type of study: Prognostic_studies Limits: Humans Language: En Journal: J Clin Microbiol Year: 2017 Document type: Article Affiliation country: Canada Country of publication: United States