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Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection.
Mitzelfelt, Katie A; McDermott-Roe, Chris; Grzybowski, Michael N; Marquez, Maribel; Kuo, Chieh-Ti; Riedel, Michael; Lai, Shuping; Choi, Melinda J; Kolander, Kurt D; Helbling, Daniel; Dimmock, David P; Battle, Michele A; Jou, Chuanchau J; Tristani-Firouzi, Martin; Verbsky, James W; Benjamin, Ivor J; Geurts, Aron M.
Affiliation
  • Mitzelfelt KA; Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA.
  • McDermott-Roe C; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: mcdec@mail.med.upenn.edu.
  • Grzybowski MN; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Marquez M; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Kuo CT; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Riedel M; PharmaCell, 6229 Maastricht, the Netherlands.
  • Lai S; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Choi MJ; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Kolander KD; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Helbling D; Division of Genetics, Department of Pediatrics, Human Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Dimmock DP; Division of Genetics, Department of Pediatrics, Human Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Battle MA; Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Jou CJ; Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; Division of Pediatric Cardiology, University of Utah School of Medicine, Salt Lake City, UT 83113, USA.
  • Tristani-Firouzi M; Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; Division of Pediatric Cardiology, University of Utah School of Medicine, Salt Lake City, UT 83113, USA.
  • Verbsky JW; Section of Quantitative Health Sciences, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Benjamin IJ; Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Geurts AM; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: ageurts@mcw.edu.
Stem Cell Reports ; 8(3): 491-499, 2017 03 14.
Article in En | MEDLINE | ID: mdl-28238794
Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genome, Human / Gene Targeting / Induced Pluripotent Stem Cells / Gene Editing Limits: Humans Language: En Journal: Stem Cell Reports Year: 2017 Document type: Article Affiliation country: United States Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genome, Human / Gene Targeting / Induced Pluripotent Stem Cells / Gene Editing Limits: Humans Language: En Journal: Stem Cell Reports Year: 2017 Document type: Article Affiliation country: United States Country of publication: United States