Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus.
Brain Struct Funct
; 222(6): 2831-2853, 2017 Aug.
Article
in En
| MEDLINE
| ID: mdl-28285370
ABSTRACT
Understanding the human brain is the ultimate goal in neuroscience, but this is extremely challenging in part due to the fact that brain tissue obtained from autopsy is practically the only source of normal brain tissue and also since changes at different levels of biological organization (genetic, molecular, biochemical, anatomical) occur after death due to multiple mechanisms. Here we used metabolomic and anatomical techniques to study the possible relationship between post-mortem time (PT)-induced changes that may occur at both the metabolomics and anatomical levels in the same brains. Our experiments have mainly focused on the hippocampus of the mouse. We found significant metabolomic changes at 2 h PT, whereas the integrity of neurons and glia, at the anatomical/ neurochemical level, was not significantly altered during the first 5 h PT for the majority of histological markers.
Key words
2-Arachidonoylglycerol; anandamide; Arginine; Aspartic acid; Astrocytes; Brain tissue; Cadaverine; Calbindin-D28K (CB); Creatine; GABA; GABAergic interneurons; GAD-65; GCMS analysis; Gangliosides; Glutamate; Glycerophosphocholine; Glycerophospholipids; Hippocampus; Histology; LCMS analysis; Metabolites; Metabolomic changes; Microglia; NADPH-diaphorase (NADPH-d) glutamatergic axon terminals; Neuronal markers; Parvalbumin; Phosphatidylcholine and phosphatidylethanolamine; Post-mortem delay; Putrescine; SMI-32
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Postmortem Changes
/
Metabolomics
/
Hippocampus
/
Neuroanatomy
Limits:
Animals
Language:
En
Journal:
Brain Struct Funct
Journal subject:
CEREBRO
Year:
2017
Document type:
Article
Affiliation country:
Spain