Optogenetic control with a photocleavable protein, PhoCl.
Nat Methods
; 14(4): 391-394, 2017 Apr.
Article
in En
| MEDLINE
| ID: mdl-28288123
ABSTRACT
To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Proteins
/
Protein Engineering
/
Optogenetics
Limits:
Humans
Language:
En
Journal:
Nat Methods
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2017
Document type:
Article
Affiliation country:
Canada