NEUROD2 Regulates Stim1 Expression and Store-Operated Calcium Entry in Cortical Neurons.
eNeuro
; 4(1)2017.
Article
in En
| MEDLINE
| ID: mdl-28303257
ABSTRACT
Calcium signaling controls many key processes in neurons, including gene expression, axon guidance, and synaptic plasticity. In contrast to calcium influx through voltage- or neurotransmitter-gated channels, regulatory pathways that control store-operated calcium entry (SOCE) in neurons are poorly understood. Here, we report a transcriptional control of Stim1 (stromal interaction molecule 1) gene, which is a major sensor of endoplasmic reticulum (ER) calcium levels and a regulator of SOCE. By using a genome-wide chromatin immunoprecipitation and sequencing approach in mice, we find that NEUROD2, a neurogenic transcription factor, binds to an intronic element within the Stim1 gene. We show that NEUROD2 limits Stim1 expression in cortical neurons and consequently fine-tunes the SOCE response upon depletion of ER calcium. Our findings reveal a novel mechanism that regulates neuronal calcium homeostasis during cortical development.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neuropeptides
/
Calcium
/
Endoplasmic Reticulum
/
Basic Helix-Loop-Helix Transcription Factors
/
Stromal Interaction Molecule 1
/
Neurons
Limits:
Animals
/
Humans
Language:
En
Journal:
ENeuro
Year:
2017
Document type:
Article
Affiliation country:
Turkey