Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modifications.
Methods Mol Biol
; 1582: 111-126, 2017.
Article
in En
| MEDLINE
| ID: mdl-28357666
ABSTRACT
Post-translational modifications (PTMs) are chemical alterations to individual amino acids that alter a protein's conformation, stability, and/or function. Several pathogenic viruses have been shown to encode proteins with PTMs, including human T-cell leukemia virus type 1 (HTLV-1) Tax and Rex regulatory proteins. HTLV-1 basic leucine zipper protein (HBZ) was hypothesized to feature PTMs due to its functional activities and interactions with cellular transcription factors and acetyltransferases. Here, we describe the approach used to identify, via mass spectrometry, the PTMs of HBZ. In addition, we describe methods to determine the functional relevance of the identified PTMs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Human T-lymphotropic virus 1
/
Protein Processing, Post-Translational
/
Retroviridae Proteins
/
Basic-Leucine Zipper Transcription Factors
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
United States