Immobilization of Enzymes on PLGA Sub-Micrometer Particles by Crosslinked Layer-by-Layer Deposition.
Macromol Biosci
; 17(8)2017 08.
Article
in En
| MEDLINE
| ID: mdl-28388000
ABSTRACT
Enzyme immobilization is of high interest for industrial applications. However, immobilization may compromise enzyme activity or stability due to the harsh conditions which have to be applied. The authors therefore present a new and improved crosslinked layer-by-layer (cLbL) approach. Two different model enzymes (acid phosphatase and ß-galactosidase) are immobilized under mild conditions on biocompatible, monodisperse, sub-micrometer poly(lactide-co-glycolide) (PLGA) particles. The resulting PLGA enzyme systems are characterized regarding their size, surface charge, enzyme activity, storage stability, reusability, and stability under various conditions such as changing pH and temperature. The developed and characterized cLbL protocol can be easily adapted to different enzymes. Potential future uses of the technology for biomedical applications are discussed. PLGA-enzyme particles are therefore injected into the blood circulation of zebrafish embryos in order to demonstrate the in vivo stability and activity of the designed system.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Polyglycolic Acid
/
Aspergillus oryzae
/
Acid Phosphatase
/
Solanum tuberosum
/
Fungal Proteins
/
Beta-Galactosidase
/
Lactic Acid
/
Enzymes, Immobilized
Language:
En
Journal:
Macromol Biosci
Journal subject:
BIOQUIMICA
Year:
2017
Document type:
Article
Affiliation country:
Switzerland
Publication country:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY