High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells.
Stem Cell Reports
; 8(4): 1101-1111, 2017 04 11.
Article
in En
| MEDLINE
| ID: mdl-28410643
ABSTRACT
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes. To date, studies have been published that use small panels of iPSC-derived cell lines to study monogenic diseases. However, to study complex diseases, where the genetic variation underlying the disorder is unknown, a sizable number of patient-specific iPSC lines and controls need to be generated. Currently the methods for deriving and characterizing iPSCs are time consuming, expensive, and, in some cases, descriptive but not quantitative. Here we set out to develop a set of simple methods that reduce cost and increase throughput in the characterization of iPSC lines. Specifically, we outline methods for high-throughput quantification of surface markers, gene expression analysis of in vitro differentiation potential, and evaluation of karyotype with markedly reduced cost.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Variation
/
Myocytes, Cardiac
/
Induced Pluripotent Stem Cells
/
High-Throughput Screening Assays
/
Karyotyping
/
Neurons
Type of study:
Health_economic_evaluation
Limits:
Humans
Language:
En
Journal:
Stem Cell Reports
Year:
2017
Document type:
Article
Affiliation country:
United States