Isolation and characterization of a thermostable F<sub>420</sub>:NADPH oxidoreductase from <i>Thermobifida fusca</i>.

Kumar, Hemant; Nguyen, Quoc-Thai; Binda, Claudia; Mattevi, Andrea; Fraaije, Marco W

Isolation and characterization of a thermostable F₄₂₀:NADPH oxidoreductase from Thermobifida fusca.

Kumar, Hemant; Nguyen, Quoc-Thai; Binda, Claudia; Mattevi, Andrea; Fraaije, Marco W.

Affiliation

Kumar H; From the Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Nguyen QT; From the Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Binda C; Scuola Universitaria Superiore IUSS Pavia, Piazza della Vittoria 15, 27100 Pavia, Italy.
Mattevi A; Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, 41 Dinh Tien Hoang St., Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam, and.
Fraaije MW; Department of Biology and Biotechnology, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy.

J Biol Chem ; 292(24): 10123-10130, 2017 06 16.

Article in En | MEDLINE | ID: mdl-28411200

Subject(s)

Actinobacteria/enzymology; Bacterial Proteins/metabolism; Models, Molecular; NADH, NADPH Oxidoreductases/metabolism; NADP/metabolism; Amino Acid Sequence; Amino Acid Substitution; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Binding Sites; Catalytic Domain; Conserved Sequence; Crystallography, X-Ray; Enzyme Stability; Hot Temperature/adverse effects; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Mutagenesis, Site-Directed; Mutation; NADH, NADPH Oxidoreductases/chemistry; NADH, NADPH Oxidoreductases/genetics; NADH, NADPH Oxidoreductases/isolation & purification; NADP/chemistry; Protein Conformation; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Structural Homology, Protein

Key words

Thermobifida fusca; actinobacteria; deazaflavin; flavin; flavoprotein; nicotinamide; oxidation-reduction (redox)

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Models, Molecular / Actinobacteria / NADH, NADPH Oxidoreductases / NADP Language: En Journal: J Biol Chem Year: 2017 Document type: Article Affiliation country: Netherlands Country of publication: United States

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