Complement C5a receptor knockout has diminished light-induced microglia/macrophage retinal migration.
Mol Vis
; 23: 210-218, 2017.
Article
in En
| MEDLINE
| ID: mdl-28442885
ABSTRACT
PURPOSE:
The complement system is involved in the pathogenesis of age-related macular degeneration (AMD). Because activated microglia are also associated with AMD, we studied the relationship between complement anaphylatoxin receptors and microglial recruitment.METHODS:
We assessed the effect of anaphylatoxin C3a receptor (C3aR) and C5a receptor (C5aR) knockout (KO) on light damage-induced migration of microglia/macrophages into the mouse outer retina via immunofluorescence and real-time quantitative PCR.RESULTS:
We found that the mRNA levels of C3, C5, C3aR, C5aR, and two activators of the complement alternative pathway, Cfb and Cfd, were all upregulated after light exposure. Retinal Iba1-positive microglia/macrophages express receptors for C3a and C5a. Light damage increased the number of retinal Iba1-positive cells and the mRNA levels of Iba1. Compared with the wild-type (WT) mice, these increases were attenuated in the C5aR KO mice but not in the C3aR KO mice.CONCLUSIONS:
C5aR but not C3aR promoted the recruitment of microglia/macrophages. These divergent properties of complement anaphylatoxins in the light damage model provide a rationale for testing the differential effects of these receptors in additional retinal and neurodegeneration models.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retinal Degeneration
/
Cell Movement
/
Microglia
/
Receptor, Anaphylatoxin C5a
/
Gene Knockout Techniques
/
Light
/
Macrophages
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Mol Vis
Journal subject:
BIOLOGIA MOLECULAR
/
OFTALMOLOGIA
Year:
2017
Document type:
Article
Affiliation country:
Panama