Chainy: an universal tool for standardized relative quantification in real-time PCR.
Bioinformatics
; 33(9): 1411-1413, 2017 05 01.
Article
in En
| MEDLINE
| ID: mdl-28453678
ABSTRACT
Summary:
Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references, standardized normalization using one or more references, and group-wise relative quantification statistical testing. We illustrate the utility of Chainy for differential expression and chromatin immunoprecipitation enrichment (ChIP-QPCR) analysis. Availability and Implementation Chainy is open source and freely available at http//maplab.cat/chainy. Contact imallona@igtp.cat. Supplementary information Supplementary data are available at Bioinformatics online.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Software
/
Chromatin Immunoprecipitation
/
Real-Time Polymerase Chain Reaction
Language:
En
Journal:
Bioinformatics
Journal subject:
INFORMATICA MEDICA
Year:
2017
Document type:
Article
Affiliation country:
Spain