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Genetic interaction mapping in mammalian cells using CRISPR interference.
Du, Dan; Roguev, Assen; Gordon, David E; Chen, Meng; Chen, Si-Han; Shales, Michael; Shen, John Paul; Ideker, Trey; Mali, Prashant; Qi, Lei S; Krogan, Nevan J.
Affiliation
  • Du D; Department of Bioengineering, Stanford University, Stanford, California, USA.
  • Roguev A; Department of Chemical and Systems Biology, Stanford University, Stanford, California, USA.
  • Gordon DE; Stanford ChEM-H, Stanford University, Stanford, California, USA.
  • Chen M; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA.
  • Chen SH; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, California, USA.
  • Shales M; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA.
  • Shen JP; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, California, USA.
  • Ideker T; Department of Bioengineering, Stanford University, Stanford, California, USA.
  • Mali P; J. David Gladstone Institutes, San Francisco, California, USA.
  • Qi LS; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA.
  • Krogan NJ; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, California, USA.
Nat Methods ; 14(6): 577-580, 2017 Jun.
Article in En | MEDLINE | ID: mdl-28481362
ABSTRACT
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and comparison with protein-protein-interaction data revealed a functional map of chromatin regulation.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromosome Mapping / Protein Interaction Mapping / Epistasis, Genetic / Clustered Regularly Interspaced Short Palindromic Repeats Limits: Animals Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2017 Document type: Article Affiliation country: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromosome Mapping / Protein Interaction Mapping / Epistasis, Genetic / Clustered Regularly Interspaced Short Palindromic Repeats Limits: Animals Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2017 Document type: Article Affiliation country: United States