Rab2 promotes autophagic and endocytic lysosomal degradation.
J Cell Biol
; 216(7): 1937-1947, 2017 07 03.
Article
in En
| MEDLINE
| ID: mdl-28483915
ABSTRACT
Rab7 promotes fusion of autophagosomes and late endosomes with lysosomes in yeast and metazoan cells, acting together with its effector, the tethering complex HOPS. Here we show that another small GTPase, Rab2, is also required for autophagosome and endosome maturation and proper lysosome function in Drosophila melanogaster We demonstrate that Rab2 binds to HOPS, and that its active, GTP-locked form associates with autolysosomes. Importantly, expression of active Rab2 promotes autolysosomal fusions unlike that of GTP-locked Rab7, suggesting that its amount is normally rate limiting. We also demonstrate that RAB2A is required for autophagosome clearance in human breast cancer cells. In conclusion, we identify Rab2 as a key factor for autophagic and endocytic cargo delivery to and degradation in lysosomes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endosomes
/
Autophagy
/
Breast Neoplasms
/
Rab2 GTP-Binding Protein
/
Drosophila Proteins
/
Drosophila melanogaster
/
Endocytosis
/
Autophagosomes
/
Lysosomes
Type of study:
Prognostic_studies
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
J Cell Biol
Year:
2017
Document type:
Article
Affiliation country:
Hungary