Monitoring of Iron Depletion-Induced Mitophagy in Pathogenic Yeast.
Methods Mol Biol
; 1759: 161-172, 2018.
Article
in En
| MEDLINE
| ID: mdl-28488243
ABSTRACT
Mitophagy, which is the degradation of mitochondria via selective autophagic machinery, is thought to be involved in regulating the mass and function of mitochondria. Methods for detection of mitophagy have been reported for several fungal cells including some budding yeast, methylotrophic yeast, and filamentous fungi. Mitophagy in Saccharomyces cerevisiae is activated under nitrogen-poor conditions; however, the regulatory mechanism of mitophagy in most fungi has not been elucidated. Here we describe methods to monitor mitophagy in the pathogenic yeast Candida glabrata under iron-depleted conditions but not under nitrogen starvation. This observation may provide some clues to elucidate the physiological roles of mitophagy in eukaryotes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Yeasts
/
Mitophagy
/
Iron
/
Mitochondria
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2018
Document type:
Article
Affiliation country:
Japan