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Decreased expression of hsa_circ_0003570 in hepatocellular carcinoma and its clinical significance.
Fu, Liyun; Wu, Shengdong; Yao, Ting; Chen, Qingqing; Xie, Yi; Ying, Sheng; Chen, Zhigang; Xiao, Bingxiu; Hu, Yaoren.
Affiliation
  • Fu L; Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.
  • Wu S; Department of Hepatology, Ningbo No. 2 Hospital and the Affiliated Hospital, Medical School of Ningbo University, Ningbo, China.
  • Yao T; Department of General Surgery, Li Hui-Li Hospital and the Affiliated Hospital, Medical School of Ningbo University, Ningbo, China.
  • Chen Q; Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.
  • Xie Y; Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.
  • Ying S; Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.
  • Chen Z; Department of Hepatology, Ningbo No. 2 Hospital and the Affiliated Hospital, Medical School of Ningbo University, Ningbo, China.
  • Xiao B; Department of Hepatology, Ningbo No. 2 Hospital and the Affiliated Hospital, Medical School of Ningbo University, Ningbo, China.
  • Hu Y; Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.
J Clin Lab Anal ; 32(2)2018 Feb.
Article in En | MEDLINE | ID: mdl-28493512
BACKGROUND: Circular RNAs (circRNAs) constitute a class of non-coding RNAs recently discovered to be widespread and abundant in mammalian cells. However, the expression features of most of circRNAs in hepatocellular carcinoma (HCC) are unraveled. In this study, we focused on hsa_circ_0003570, which was found to be down-regulated in HCC tissues in our previous microarray screening. METHODS: The hsa_circ_0003570 levels in HCC cell lines, HepG2, SMMC-7721, MHCC97L, MHCC97H, and HCCLM3, and human normal hepatic cell line L02 were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Then, its levels in 107 paired HCC tissues and adjacent non-tumor tissues, 60 liver biopsy samples from patients with chronic liver diseases were detected by qRT-PCR. The receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of hsa_circ_0003570 for HCC. RESULTS: Hsa_circ_0003570 was not only first found down-regulated in HCC cell lines (P<.001) but also in HCC tissues (P<.001). Moreover, hsa_circ_0003570 was gradually decreased from chronic hepatitis (CH), to liver cirrhosis (LC) and to HCC tissues (P<.01). Its expression levels were significantly correlated with tumor diameter (P=.035), differentiation (P=.013), microvascular invasion (P=.045), Barcelona Clinic Liver Cancer stages (P=.011), tumor-node-metastasis stages (P=.016), and serum alpha-fetoprotein levels (P=.031). The ROC curve demonstrated that hsa_circ_0003570 had poor performance for differentiating HCC from LC and CH, but had relatively good performance for differentiating LC from CH. CONCLUSIONS: These results indicated that hsa_circ_0003570 expression levels were associated with HCC clinicopathological characteristics.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA / Biomarkers, Tumor / Carcinoma, Hepatocellular / Liver Neoplasms Type of study: Prognostic_studies Limits: Female / Humans / Male / Middle aged Language: En Journal: J Clin Lab Anal Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2018 Document type: Article Affiliation country: China Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA / Biomarkers, Tumor / Carcinoma, Hepatocellular / Liver Neoplasms Type of study: Prognostic_studies Limits: Female / Humans / Male / Middle aged Language: En Journal: J Clin Lab Anal Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2018 Document type: Article Affiliation country: China Country of publication: United States